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pCMV-T7 RNA POL

Plasmid

#138352

Purpose

Expressing T7 RNA Polymerase in mammalian cells

Depositor

Baisong Lu

Insert

T7 RNA POL

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV

Plasmid

#79121

Purpose

Lentiviral vector for constitutive expression of genes (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-Sumo1 WT

Plasmid

#48966

Purpose

Expresses HA-Sumo1 WT in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-mitoDsRed

Plasmid

#44386

Purpose

3rd generation lentiviral vector

Depositor

Pantelis Tsoulfas

Article

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoDsRed

Use

Lentiviral

Tags

61 aa targeting sequence of the P1 isoform F1F0-A…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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spNW15

Plasmid

#173483

Purpose

Expresses spNW15 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW15

Use

Tags

Histag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GST-MNase

Plasmid

#136291

Purpose

Expresses Micrococcal nuclease

Depositor

Kavitha Sarma

Article

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-ARP2-N-14

Plasmid

#54980

Purpose

Localization: Actin Related Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
VAMP2-pHmScarlet

Plasmid

#166890

Purpose

For vesicle exocytosis visualization using pHmScarlet

Depositor

Pingyong Xu

Article

Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

Insert

VAMP2 (Vamp2 Mouse)

Use

Tags

pHmScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225239

Purpose

Mammalian expression of iPASnFR-H in mitochondria

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Use

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs GFP-LC3-RFP

Plasmid

#117413

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RLUC POLIRES FLUC

Plasmid

#45642

Purpose

Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

Depositor

Nahum Sonenberg

Article

Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

Insert

RLUC - IRES - FLUC

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-12X

Plasmid

#27119

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (12)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST PUM2

Plasmid

#40292

Depositor

Thomas Tuschl

Article

Hafner et al Cell. 2010 Apr 2;141(1):129-41.

Insert

Pumilio homolog 2 (PUM2 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HBM-Luc

Plasmid

#35155

Depositor

Linda Penn

Article

Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

Insert

Myc promoter (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains 2.5 kb of the c-myc minimal promoter

Promoter

c-myc

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Use

Tags

ratiometric pHluorin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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