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Plasmid#154292PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationD554N mutation (Ser/Thr glycosylation-incompetent)PromoterPGKAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT 5N5A (Puro)
Plasmid#154293PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationN322A, N356A, N390A, N424A, N458A mutations; HCF-…PromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-iRFP670
Plasmid#110694PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670DepositorInsertCPSF6-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationTruncation of CPSF6 isoform at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo E240R
Plasmid#207345PurposeBacterial expression of LRRK2 ArmadilloDepositorInsertLRRK2 Armadillo (LRRK2 Human)
UseTags2xHisExpressionBacterialMutationE240R; Armadillo domain only (aa 1-552)PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo K439E
Plasmid#207346PurposeBacterial expression of LRRK2 ArmadilloDepositorInsertLRRK2 Armadillo (LRRK2 Human)
UseTags2xHisExpressionBacterialMutationK439E; Armadillo domain only (aa 1-552)PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo F283A
Plasmid#207348PurposeBacterial expression of LRRK2 ArmadilloDepositorInsertLRRK2 Armadillo (LRRK2 Human)
UseTags2xHisExpressionBacterialMutationF283A; Armadillo domain only (aa 1-552)PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag-LRRK2 K17A/K18A/R1441G
Plasmid#186013PurposeFlag-LRRK2 K17A/K18A/R1441GDepositorInsertFlag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
UseTagsExpressionMammalianMutationSee depositor's comments below.PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 1
Plasmid#198489Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 1DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 2
Plasmid#198490Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 2DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mAtp6v1a-1
Plasmid#198477Purposelentiviral stable expression of mAtp6v1a gRNA 1DepositorInsertmouse ATPv1a (Atp6v1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mAtp6v1a-2
Plasmid#198478Purposelentiviral stable expression of mAtp6v1a gRNA 2DepositorInsertmouse ATPv1a (Atp6v1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-4Glu-iRFP670
Plasmid#167588PurposeExpresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670DepositorInsertCPSF6-4Glu-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationK547E, R549E, R559E, R561EPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRIG-myc-Sema4D-CD4
Plasmid#51606Purposeexpresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backboneDepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseLentiviralTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterRSVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only