-
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
UseTags6x His tag, FLAG tag, SUMO tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 FL (1-440)
Plasmid#210425PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage siteDepositorInsertp62 FL (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+18)
Plasmid#199162Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+18)
UseTagsMGHHHHHHGGAExpressionMutationE16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)
Plasmid#199160Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+6)
UseTagsMGHHHHHHGGAExpressionMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC
Plasmid#99641PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
UseTagsMGHHHHHHGGAExpressionMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JT113-pETDuet1-(R)-hREV3L
Plasmid#64872Purposebacterial expression of human REV3LDepositorInsertREV3L (REV3L Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-30)
Plasmid#199167Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(-30)
UseTagsMGHHHHHHGGAExpressionMutationT19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp9-His
Plasmid#11829DepositorInsertCaspase 9 (CASP9 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+24)
Plasmid#199163Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+24)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28c(+)-YTHDF2
Plasmid#102275PurposeFor bacterial expression of His-tagged human YTHDF2DepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-HA2
Plasmid#165991PurposeSpyCatcher-fused influenza HA ministem with removable HisTagDepositorInsertSpyCatcher-HA2
UseTagsFoldon, HisTag, and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_05
Plasmid#194666PurposeProtein production of the split HaloTag based calcium recorder Caprola_05 in bacteriaDepositorInsertCaprola_05
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp6-His
Plasmid#11823DepositorInsertCaspase 6 (CASP6 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDBamHI-Leu
Plasmid#38256DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInorganic pyrophosphatase (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only