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Plasmid#117528PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.1DepositorInsertSp-eCas9 1.1 (with stop codon)
UseCRISPRTagsExpressionMutationK848A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0015
Plasmid#117525PurposeLevel0 Golden Gate part: CDS, SpCas9-KA (no stop codon)DepositorInsertSpCas9-KA (no stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0014
Plasmid#117524PurposeLevel0 Golden Gate part: CDS, SpCas9-KADepositorInsertSpCas9-KA (with stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0001
Plasmid#117523PurposeLevel0 Golden Gate part: CDS, SpCas9-DE (no stop codon)DepositorInsertSpCas9-DE (no stop codon)
UseCRISPRTagsExpressionMutationD1135EPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0009
Plasmid#117522PurposeLevel0 Golden Gate part: CDS, SpCas9-p (no stop codon)DepositorInsertSpCas9-p (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL90005
Plasmid#117520PurposeLevel0 Golden Gate part: CDS, SpCas9-h (no stop codon)DepositorInsertSpCas9-h (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0008
Plasmid#117535PurposeLevel0 Golden Gate part: CDS, AsCas12aDepositorInsertAsCas12a (with stop codon)
UseCRISPRTags3xHA and NLSExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF004
Plasmid#48845PurposeProvides a D-alanine resistance cassette as GreenGate module.DepositorInsertp35S::D-AlanineR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition site in 35S promoter re…PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B
Plasmid#69283PurposeGolden Gate entry vector; 3rd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B
Plasmid#69281PurposeGolden Gate entry vector; 1st gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B
Plasmid#69282PurposeGolden Gate entry vector; 2nd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B
Plasmid#179216PurposeGolden Gate entry vector; 4th gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAtU3Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM246 - mosTI - unc-119 - MCS
Plasmid#182343PurposeEmpty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDV002
Plasmid#191823PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV044
Plasmid#191824PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only