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Plasmid#79869PurposeExpresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.DepositorInsertGFP-GFE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
Plasmid#134612PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 375 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13A tail 361-1749
Plasmid#134622PurposeMammalian expression of mouse KIF13A 361-1749DepositorInsertKIF13A (Kif13a Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 360 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-6xgata2aECEbasp
Plasmid#132968Purposegata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vectorDepositorInsertgata2a endothelial enhancer (x6) with a b-actin basal promoter
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterChicken B-ActinAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB.GFP-KIF1A tail 395-1698
Plasmid#134618PurposeMammalian expression of mouse KIF1A 395-1698DepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 394 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2-1
Plasmid#31828DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
Plasmid#52890Purpose"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.DepositorInsertsUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13B tail 442-1843
Plasmid#134623PurposeMammalian expression of mouse KIF13B 442-1843DepositorInsertKIF13B (Kif13b Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 441 N-terminal residues.PromoterChicken Beta actinAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-RandE3
Plasmid#79870PurposeExpresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.DepositorInsertGFP-RandE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF1C tail 400-1103
Plasmid#134621PurposeMammalian expression of human KIF1C 400-1103DepositorInsertKIF1C (KIF1C Human)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B tail 391-747
Plasmid#134615PurposeMammalian expression of mouse KIF3B 391-747DepositorInsertKIF3B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 390 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mBAF45b IRES GFP
Plasmid#17881DepositorInsertBAF45b (Dpf1 Mouse)
UseTagsFLAG and IRES EGFPExpressionMammalianMutationPromoterAvailable sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C tail 411-796
Plasmid#134616PurposeMammalian expression of mouse KIF3C 411-796DepositorInsertKIF3C (Kif3c Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 410 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-EB3-NeonGreen
Plasmid#175263Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3 (MAPRE3 Human)
UseTagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorInsertRFPnols-PQR-ZeocinR (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF17 tail 400-1038
Plasmid#134617PurposeMammalian expression of mouse KIF1A 400-1038DepositorInsertKIF17 (Kif17 Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only