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mCardinal-Lifeact-7

Plasmid

#54663

Purpose

Localization: Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Use

Tags

mCardinal-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pJR025

Plasmid

#198832

Purpose

Actin filament and cell cortex localization.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::act2-venus::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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2xFKBP-mCh-KDEL

Plasmid

#173010

Purpose

FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

2xFKBP-mCh-KDEL

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36880

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

tTA2
tdT-3Myc
insulator

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF1Bbeta tail 387-1770

Plasmid

#134620

Purpose

Mammalian expression of mouse KIF1Bbeta 387-1170

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Bbeta (Kif1b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF1Balpha tail 387-1150

Plasmid

#134619

Purpose

Mammalian expression of mouse KIF1Balpha 387-1150

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Balpha (Kif1b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCIG-VP16–RAR-alpha

Plasmid

#80434

Purpose

Expression of constitutively active human RAR-alpha in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Retinoic acid receptor alpha (RARA Human)

Use

Tags

EGFP and VP16

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

Plasmid

#175261

Purpose

labelling of microtubule plus end and F-actin structures

Depositor

Sebastián Dupraz, Frank Bradke

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)

Use

Tags

mCherry (on iLifeact) and mNeonGreen (on EB3)

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS mSynCAM1

Plasmid

#115496

Purpose

expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm1 (Cadm1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only
pQCXIH BRGI-Flag

Plasmid

#19148

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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DHFR-mNeon-GluA1

Plasmid

#173009

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-mNeon-GluA1

Use

Tags

DHFR and mNeon

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV CAG ChR2 E123T T159C 2A tDimer

Plasmid

#85399

Purpose

High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E123T , T159C

Promoter

CAG and ribosomal skip sequence 2A

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA1

Plasmid

#82543

Purpose

Overexpression vector for Venus-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Use

Tags

Venus fluorescent protein

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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inhibin-DsRed

Plasmid

#156184

Purpose

Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

Depositor

Goro Yoshizaki

Article

Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

Inserts

rainbow trout inhibin α promoter
rainbow trout β actin 3'UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

inhibin

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA2

Plasmid

#82544

Purpose

Overexpression vector for Venus-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Use

Tags

Venus fluorescent protein

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

Plasmid

#132685

Purpose

AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 2 (Cndp2 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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