Addgene

We narrowed to 22,673 results for: GFP

Showing: 1581 - 1600 of 22673 results

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-RandE3

Plasmid

#79870

Purpose

Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro1GFP

Plasmid

#82365

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro3GFP

Plasmid

#82367

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro3GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/N149C/S202C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro4GFP

Plasmid

#82368

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro4GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/N149C/S202C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFPTET1CD

Plasmid

#82561

Purpose

Expression vector for antibody-sfGFP-TET1CD.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

scFvGCN4sfGFPTET1CD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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p3E-GFP-HA no-pA

Plasmid

#82589

Purpose

3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_H2B

Plasmid

#82605

Purpose

Expresses sfCherry2(11) tagged H2B in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_H2B

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_HP1

Plasmid

#82607

Purpose

Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_HP1b

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Availability

Academic Institutions and Nonprofits only
pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Use

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

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Fv-hCaspase 8-2A-GFP

Plasmid

#82712

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

LTR

Availability

Academic Institutions and Nonprofits only

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Fv-hCaspase 8 (C/A)-2A-GFP

Plasmid

#82713

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8 (C/A)-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiex-1 AC30_eGFP

Plasmid

#82715

Purpose

Expresses a GFP-tagged recombinant adenylyl cyclase.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)

Use

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

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pGH311_wtGFP S65T

Plasmid

#85408

Purpose

lentiviral expression vector for wtGFP S65T

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pGH312_wtGFP Q80H

Plasmid

#85409

Purpose

lentiviral expression vector for wtGFP Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP Q80H

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP

Plasmid

#85482

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Arabinose

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP

Plasmid

#85492

Purpose

pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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