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Plasmid#176933PurposeExpresses PE2-NG variant in mammalian cellsDepositorInsertPE2-NG variant
UseLentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEFSAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP YAP1
Plasmid#193675PurposeConstitutive lentiviral expression of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
negMaLionR
Plasmid#113909Purposenegative control for Red ATP sensorDepositorInsertnegMaLionR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
Plasmid#64218PurposeExpression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-BFP-P2A-E1BExpressionMammalianMutationPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
FlucSM-HA-GFP11-N1
Plasmid#91955PurposeExpresses aggregation-prone FlucSM-HA-GFP11 in mammalian cellsDepositorInsertFlucSM-HA-GFP11
UseTagsExpressionMammalianMutationR188QPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVβ-p300.DY-myc
Plasmid#30490DepositorInsertp300 (EP300 Human)
UseTagsMycExpressionMammalianMutationaspartic acid 1399 converted to tyrosine (Acetyla…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of FireFly luciferase stop codon between the ORF and MCS and polyADepositorInsertFireFly luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Peredox
Plasmid#73807PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)DepositorInsertPeredox
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-mRFP1
Plasmid#53745PurposeContains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vectorDepositorInserthistone H2B (H2BC21 Human)
UseTagsmRFPExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF
Plasmid#82021PurposecLac143; pALM143; IPTG inducible systemDepositorInsertYFP
UseTagsExpressionMutationPromoterPcptoo_C38T,A39TAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-300(HAT-)
Plasmid#23254DepositorInsertp300 (EP300 Human)
UseTagsExpressionMammalianMutationH1415A E1423A Y1424A L1428S Y1430A H1434APromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CDK4
Plasmid#116724PurposeLentiviral expression of CDK4DepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
FlucDM-HA-GFP11-N1
Plasmid#91956PurposeExpresses aggregation-prone FlucDM-HA-GFP11 in mammalian cellsDepositorInsertFlucDM-HA-GFP11
UseTagsExpressionMammalianMutationR188Q,R261QPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a_SUMO_AcCas12n
Plasmid#203808PurposepET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.DepositorInsertSUMO_AcCas12n
UseCRISPRTagsExpressionMutationPromoterT7Available sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTF102
Plasmid#134774PurposeA binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-ICP0-C3
Plasmid#134559PurposeExpresses YFP-ICP0 fusion proteinDepositorInsertICP0
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 ORF7A
Plasmid#141276PurposeGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPCXU6PT7SK
Plasmid#27549DepositorInsertU6PT7SK (RN7SK Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAGM51323
Plasmid#153224PurposeLevel 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminatorDepositorInsertArabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMito.iGABASnFR
Plasmid#112175PurposeGABA SensorDepositorInsertiGABASnFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRMT8-FLAG
Plasmid#164699PurposeExpression of PRMT8 with a C-terminal FLAG tagDepositorInsertPRMT8 (PRMT8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dSpRY-Act3.0
Plasmid#158416PurposeCRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRmRuby2
Plasmid#59148PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL2-pCMV7.1
Plasmid#155020PurposeN-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vectorDepositorInsertCullin 2 (CUL2 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TGT-His
Plasmid#138201PurposeExpresses E. Coli TGT with a C-terminal His TagDepositorInsertE Coli tRNA Guanine Transglycosylase-His
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Golgi-mEGFP
Plasmid#182877PurposeLocalization: Gal-T - Golgi ComplexDepositorInsertGolgi-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-targeted FRB (LDR)
Plasmid#20147DepositorInsertLyn-linker-FRB (LDR) (MTOR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmiRA1-MYB
Plasmid#141120PurposeLentiviral overexpression of MYB in mammalian cells.DepositorInsertMYB (MYB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hFrataxin-HA
Plasmid#31895DepositorInsertFrataxin (FXN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only