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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pv9_MBD1_MBD-R22A-13XL-BASU

Plasmid

#179417

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1.MBD-R22A-13XL-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

Plasmid

#179402

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd(DW890/891AA)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Phd domain DW890/891AA

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD

Plasmid

#179407

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ASAP3-WPRE

Plasmid

#132318

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3Kv-WPRE

Plasmid

#132332

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3Kv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R106W

Plasmid

#179408

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R106W) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R106W

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R133C

Plasmid

#179409

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R133C) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R133C

Promoter

CAGGS

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-T158M

Plasmid

#179410

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(T158M) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD T158M

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ASAP3Kv-WPRE

Plasmid

#132330

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3KV

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#153362

Purpose

Converts DNA sequences into Phytobricks

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 G7t

Plasmid

#153380

Purpose

Agrobacterium terminator for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G7 terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 HptII

Plasmid

#153391

Purpose

Confers resistance to hygromycin

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

HptII

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 NptII

Plasmid

#153389

Purpose

Confers resistance to kanamycin and G418

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

NptII

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 AlcR

Plasmid

#153410

Purpose

Ethanol inducible system trans activator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

AlcR CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 sXVE

Plasmid

#153406

Purpose

Estradiol inducible system trans activator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

sXVE CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pToneDest

Plasmid

#67691

Purpose

attR4-attR3 Destination cassette in Tol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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lvl0 FAD2t

Plasmid

#153377

Purpose

Plant terminator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

FAD2 terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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