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Plasmid#227085PurposeBacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3747_GST-TEV-Spt6p-tSH2-1223-1452
Plasmid#227086PurposeBacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationR1282HPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3736_GST-TEV-Spt6p-tSH2-1223-1454
Plasmid#227088PurposeBacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationR1282H, K1355A, K1435APromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268
Plasmid#227089PurposeBacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)DepositorInserttom1p-HECT (TOM1 Budding Yeast)
UseTagsHIS-MBP-TEV_cleavage_siteExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG
Plasmid#217324PurposePurification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
UseTagsExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG
Plasmid#217321PurposePurification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
371-B
Plasmid#97027PurposeHis6-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneUseTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-Bc
Plasmid#97028PurposeyORF-tev-His6DepositorTypeEmpty backboneUseTagsHis6 tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-T
Plasmid#97035PurposeHis6-Thioredoxin-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, TEV cleavage site, and Thioredoxin funs…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-R
Plasmid#97033PurposeStrepII-tev-yORFDepositorTypeEmpty backboneUseTagsStrepII tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-S
Plasmid#97034PurposeHis6-Sumo-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, SUMO domain, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-O
Plasmid#97032PurposeHis6-Mocr-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, TEV cleavage site, and mOCR fusionExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-L
Plasmid#97031PurposeFLAG-tev-yORFDepositorTypeEmpty backboneUseTagsFLAG tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-C
Plasmid#97029PurposeHis6-MBP-N10-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, MBP domain, N10 linker, and TEV cleavag…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_S235A
Plasmid#40639DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…ExpressionBacterialMutationS235APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM.004
Plasmid#228690PurposeC terminal dual protein A affinity tag with TEV cleavage siteDepositorInsertTEV-2xProtein A
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only