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GGE151

Plasmid

#120751

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_4UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_4UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GGE150

Plasmid

#120750

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_2UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_2UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GGE294

Plasmid

#120760

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_4UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P3_4UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GGE146

Plasmid

#120742

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_4UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_4UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GGE317

Plasmid

#120761

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_8UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P3_8UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GGE147

Plasmid

#120743

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_8UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_8UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE145

Plasmid

#120741

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_2UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_2UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GGE292

Plasmid

#120759

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_2UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P3_2UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00396

Plasmid

#34507

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8961 (tef Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pHS2

Plasmid

#81037

Purpose

HO endonuclease expression

Depositor

John McCusker

Insert

HYGMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Insert

Promoter TEF

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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natMX6-ins5

Plasmid

#195042

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

NatR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TRP1MX6-ins4

Plasmid

#195041

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

TRP1

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSHUT4-eYFP

Plasmid

#133094

Purpose

Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

Depositor

Jens Laurids Sørensen

Article

Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

Insert

enhanced yellow flourescent protein

Use

Fungal expression

Tags

Expression

Mutation

Promoter

TEF-1α

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins2

Plasmid

#195039

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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his3MX6-ins3

Plasmid

#195040

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

His5+

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins6

Plasmid

#195043

Purpose

pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0718

Plasmid

#141671

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TEF (TEF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins1

Plasmid

#195038

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Gal4-TEAD4

Plasmid

#24640

Depositor

Kunliang Guan

Insert

TEAD-4 (TEAD4 Human)

Use

Tags

Gal4

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only