-
Plasmid#125765PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK-Sa1 promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBMCS-SortA
Plasmid#21931DepositorInsertsortase (srtA Staphylococcus aureus (strain N315))
UseTagsGB1 and His-tagExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL0_9 [pUB110 ORI]
Plasmid#198924PurposeLevel 0 partDepositorInsertpUB110 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti
Plasmid#138315PurposeLenti expression of mTgBFP2DepositorInsertmTAGBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH1
Plasmid#84451Purposeshuttle plasmid for sarA P1-dsRED expressionDepositorInsertDsRed.T3(DNT)
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID
Plasmid#188653PurposeExpresses SaAID in mammalian cellsDepositorInsertnSaCas9-AID-UGI
UseTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterpCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBHPS-SortA
Plasmid#21930DepositorInsertsortase
UseTagsGB1, HRV3C cleagage site, and HisExpressionBacterialMutationdeleted amino acids 1-59PromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD009
Plasmid#116850PurposeExpresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009DepositorInsertsAraC
alpha-HL
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-PuroR_lenti
Plasmid#138317PurposeLenti expression of SpyCas9 with Puro selectionDepositorInsertSpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
SAPDF-pET21a(+)
Plasmid#12649DepositorInsertSA Peptide deformylase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0021
Plasmid#117532PurposeLevel0 Golden Gate part: CDS, SaCas9 (no stop codon)DepositorInsertSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti
Plasmid#138312PurposeLenti expression of AcrIIA14, C-terminusDepositorInsertAcrIIA14, C terminus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPhygSaCas9
Plasmid#123262PurposeExpresses Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorInsertStaphylococcus aureus Cas9 (NEWENTRY )
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0012
Plasmid#117533PurposeLevel0 Golden Gate part: CDS, eSaCas9 (no stop codon)DepositorInserteSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationR499A,Q500K,R654A,G655RPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. aureus scaffold CARGO array
Plasmid#191317PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-pAG-TM
Plasmid#207318PurposeFunctional incorporation of protein AG (pAG) fusion protein into envelope of virionsDepositorInsertprotein AG
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorArticleInsertHalotag-CNA35
UseTagsHalotagExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only