Skip to main content
Addgene
Showing: 141 - 160 of 201 results

  2. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid
    #131137
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Use
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131137-plasmid-map-sequence-id-258861

    Enlarge

  3. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid
    #82473
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Article
    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d
    Insert
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
    Use
    Tags
    TEV-Biotin and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82473-plasmid-map-sequence-id-163076

    Enlarge

  4. FCM101

    Plasmid
    #194298
    Purpose
    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
    Depositor
    Shohei Koide
    Article
    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:
    Insert
    FCM101
    Use
    Tags
    His tag, FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    194298-plasmid-map-sequence-id-381074

    Enlarge

  8. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

    Enlarge

  9. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

    Enlarge

  10. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

    Enlarge

  11. PTS

    Plasmid
    #39015
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTS (PTS Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pCRII-Topo Lhx2

    Plasmid
    #45873
    Depositor
    Jeffrey Macklis
    Insert
    Lhx2 in situ probe (Lhx2 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1809-2217 of NM_010710.3
    Promoter
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pCS6-mPSD95a-TSa-HA

    Plasmid
    #42235
    Purpose
    TimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPa cassette used for for low abundance proteins or proteins with slow ongoing synthesis rates
    Depositor
    Roger Tsien
    Article
    Lin et al Curr Protoc Protein Sci. 2010 Feb;Chapte
    Insert
    PSD95
    Use
    Tags
    TSa-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    42235-plasmid-map-sequence-id-57041

    Enlarge

  16. pCS6-mPSD95a-TSt-HA

    Plasmid
    #42237
    Purpose
    TimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis rates
    Depositor
    Roger Tsien
    Article
    Lin et al Curr Protoc Protein Sci. 2010 Feb;Chapte
    Insert
    PSD95
    Use
    Tags
    TSt-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    42237-plasmid-map-sequence-id-57043

    Enlarge

  17. KCTD13A

    Plasmid
    #74654
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCTD13A (KCTD13 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 160 of 201 results