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RP1B FUS 1-163 S12xQ

Plasmid

#127193

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 S12xQ (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2441

Plasmid

#142079

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2440

Plasmid

#142944

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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TH0901-pOCC177-FUS-wt_opt(Nhe)

Plasmid

#221885

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJ411 FUS 1-163 Y24xF

Plasmid

#127196

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 Y24xF (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Y24xF: Y6F, Y14F, Y17F, Y25F, Y33F, Y38F, Y41F, Y…

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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GST-FUS 465-526

Plasmid

#44983

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (465-526aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 465-526

Promoter

tac

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
GST-FUS 266-526

Plasmid

#44981

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (266-526aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 266-526

Promoter

tac

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
GST-FUS 356-526

Plasmid

#44982

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (356-526aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 356-526

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
p426-Cup1p-FUS-FusionRed

Plasmid

#188393

Purpose

Cu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FUS (FUS Human)

Use

Tags

FusionRed

Expression

Yeast

Mutation

Promoter

CUP1 promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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GST-FUS ΔNLS 1-513

Plasmid

#44984

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS ΔNLS (1-513aa) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 1-513

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
TH1150-pOCC177-FUS(Nhe)-PLD_YtoF_RGG_RtoK

Plasmid

#221889

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In PLD all Tyr (Y) changed to Phe (F), in RGG (aa…

Promoter

PH promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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TH0994-pOCC177-Fus-wt_(1-211)

Plasmid

#221886

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

PLD domain (aa 1-211)

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TH1148-pOCC177-FUS(Nhe)-PLD_YtoF

Plasmid

#221887

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In PLD all Tyr (Y) changed to Phe (F)

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TH1149-pOCC177-FUS(Nhe)-RGG_RtoK

Plasmid

#221888

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

In RGG (aa 212-526) most of Arg (R) changed to Ly…

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FUS_FLI1

Plasmid

#205822

Purpose

Express mEGFP-tagged fusion protein, FUS_FLI1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

FUS_FLI1 (FUS, FLI1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge