Skip to main content
Addgene
Showing: 141 - 160 of 683 results

  2. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid
    #164903
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
    Depositor
    Takeshi Imai
    Article
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y40C mutation in the Rac1 domain whcih make the s…
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164903-plasmid-map-sequence-id-340567

    Enlarge

  5. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid
    #100281
    Purpose
    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Use
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa)
    Promoter
    CAG promoter
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100281-plasmid-map-sequence-id-197245

    Enlarge

  8. pCAGGS-pac-T2A-iCre (v2)

    Plasmid
    #125821
    Purpose
    Puromycin resistance gene and iCre recombinase (version 2)
    Depositor
    Mario Capecchi
    Insert
    pac-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    125821-plasmid-map-sequence-id-247197

    Enlarge

  9. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Use
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    117699-plasmid-map-sequence-id-368109

    Enlarge

  10. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid
    #113837
    Purpose
    Monomeric TagBFP2 and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113837-plasmid-map-sequence-id-222246

    Enlarge

  12. pCAGGS-mTagBFP2-T2A-iCre (v2)

    Plasmid
    #125822
    Purpose
    Monomeric TagBFP2 and iCre recombinase (version 2)
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    125822-plasmid-map-sequence-id-247153

    Enlarge

  13. pCAGGS-mTagBFP2-T2A-FLPo

    Plasmid
    #124834
    Purpose
    Monomeric TagBFP2 and FLP recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-FLPo
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    124834-plasmid-map-sequence-id-247146

    Enlarge

  18. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid
    #100282
    Purpose
    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    Use
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-621 aa)
    Promoter
    CAG promoter
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100282-plasmid-map-sequence-id-197258

    Enlarge

  19. pCAGGS-pac-T2A-FLPo

    Plasmid
    #124835
    Purpose
    Puromycin resistance gene and FLP recombinase
    Depositor
    Mario Capecchi
    Insert
    pac-T2A-FLPo
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    124835-plasmid-map-sequence-id-247151

    Enlarge

  20. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid
    #100540
    Purpose
    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    Use
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa), Y276H muta…
    Promoter
    CAG promoter
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100540-plasmid-map-sequence-id-197261

    Enlarge

Showing: 141 - 160 of 683 results