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Plasmid#100913Purposedeletes synpcc7942_1377 (spoT)DepositorInsertKanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC
Plasmid#101828Purposedrives expression of kaiBC from an rpaA-independent promoterDepositorInsertkanamycin casette and p0050 promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::glgP_gap1
Plasmid#101845PurposeReplaces native promoter of glgP_gap1 with Ptrc promoterDepositorInsertCmR casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::zwf_fbp_opcA
Plasmid#101846PurposeReplaces native promoter of zwf_fbp_opcA with Ptrc promoterDepositorInsertsp/st resistance casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA
Plasmid#101830Purposedrives expression of rpaA from an rpaA-independent promoterDepositorInsertRpaA with p0050 promoter (+ gentamicin resistance casette)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::gnd
Plasmid#101844Purposedrives expression of gnd using Ptrc promoterDepositorInsertKan casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
zwf-fbp-opcA-
Plasmid#101839Purposedeletes zwf_fbp_opcA locusDepositorInsertKanamycin casette (in place of synpcc7942_2333-2335 operon)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP969
Plasmid#98802PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFHS-TEV T146S, D148P, S153N, S170A, N177M
UseTagsExpressionBacterialMutationT146S, D148P, S153N, S170A, N177MPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP824
Plasmid#98803PurposeExpression VectorDepositorInsertpET28 MBP-ENLYGQS-TEV V209M, W211I, M218F
UseTagsExpressionBacterialMutationV209M, W211I, M218FPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVD30
Plasmid#63691PurposepCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.DepositorInsertV. cholera chromosomal DNA
UseTagsExpressionBacterialMutation540 bp of CT A is deletedPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0037
Plasmid#181934PurposeDual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)DepositorInsertsGaussia luciferase
Cypridina luciferase
UseTagsExpressionMammalianMutationPromoterCMV and EF1alphaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR.DT-A
Plasmid#35955DepositorInsertdiphtheria toxin A
UseBac recombineeringTagsExpressionMutationPromoterPGKAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH625_pJH_LldR_PlldR-sfGFP.0TAG
Plasmid#163724PurposeLactate-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRα
Plasmid#53731PurposePlpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertαNTD
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Flag
Plasmid#17364DepositorInsertMMLV proviral DNA
UseTagsFlagExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA (KmR)
Plasmid#102321Purposedrives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)DepositorInsertRpaA with p0050 promoter (+ kanamycin resistance casette)
UseCloning vectorTagsExpressionMutationPromoter-Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only