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Plasmid#141102Purposebioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosisDepositorInsertSEP-Nanoluc
UseLentiviralTagsExpressionMutationPromoterEF1Ī±Available sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-amylase sensor
Plasmid#186293PurposeExpression alpha-amylase sensor in e coliDepositorInsertcpLumiLuc-amylase sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F2-empty vector
Plasmid#125552PurposeN2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 alone without any attached protā¦ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, aā¦Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F1-empty vector
Plasmid#125551PurposeN2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 alone without any attached protā¦ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, aā¦Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT447
Plasmid#139387PurposeBile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmRDepositorInsertBile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-EF1a-NLuc-IRES-Puro
Plasmid#130936PurposePiggyBac vector for constitutive NanoLuc expression in mammalian cellsDepositorInsertNanoLuc
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdBIP1proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[TEV]
Plasmid#200122PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
UseTagsExpressionBacterialMutationPromoterPcphy23Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK20
Plasmid#210582PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdGAPDHproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdH3proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pEK21
Plasmid#210586PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdTUBA1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdTUBA1proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK23
Plasmid#210595PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH4pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdH4proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK24
Plasmid#210597PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdACTINpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdACTINproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
NK809
Plasmid#196406PurposeStrong expression of firefly and nanoluc luciferases codon-optimized for S. rosettaDepositorInsertSro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK810
Plasmid#196407PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly controlDepositorInsertSro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only