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Plasmid#169537PurposeTier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)DepositorInsertPCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-mRuby2-4x128-mAGNET
Plasmid#166021PurposeExpresses mRuby in CaMKII+ cells with low miRNA-128 expressionDepositorInsertmRuby2 with 4 miR-128 binding sites
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW770_pCAG-PV4-loxP-mRuby2-BGHpA
Plasmid#87577PurposePV4 for 2-input, 4-output decoder circuitDepositorInsertmRuby2
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-PKIalpha-IRES-nls-mRuby2
Plasmid#63059PurposeExpresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertPKIalpha (Pkia Synthetic, Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL
Plasmid#111389PurposeAAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsChETA-mRuby2
W3SL
UseAAVTagsmRuby2ExpressionMammalianMutationW3SL is built from a shortened WPRE, an upstream …PromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2
Plasmid#113885PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-Kv-P2A-H2b-mRuby2
Plasmid#89570PurposeChannelrhodopsin localized to neuronal soma with simultaneous nuclear labelingDepositorInsertsChannelrhodopsin
Histone 2B
UseAAVTagsmRuby2ExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2
Plasmid#113889PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2DepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-MRLC1-mRuby2-IRES-PuroR
Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-tmeGFP-IRES-nls-mRuby2
Plasmid#63060PurposeExpresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertnls-mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#50942PurposeBicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA
Plasmid#51473PurposeBicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6m
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40
Plasmid#124603PurposeCre-dependent soma-localized ChrimsonR for optogenetic stimulationDepositorHas ServiceAAV9InsertSoma-localized ChrimsonR
UseAAVTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#68719PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6f
UseAAVTagsExpressionMutationPromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL
Plasmid#111390PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mRuby2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#68717PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationCre-dependent expression from inverted open readi…PromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
Plasmid#68720PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6s
UseAAVTagsExpressionMutationCre-dependent expression from inverted open readi…PromoterhSyn1-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL
Plasmid#111391PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mRuby2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
Plasmid#124604PurposeCre-dependent soma-localized C1V1(t/t) for optogenetic stimulationDepositorInsertSoma-localized C1V1(t/t)
UseAAVTagsmRuby2ExpressionMammalianMutationPromoterEf1aAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only