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pLEX-TCRb-FKBP-Blasticidin

Plasmid

#120723

Purpose

Expresses ER-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

T cell receptor β chain

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Raf1RBD

Plasmid

#120707

Purpose

Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Raf1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Raf1 amino acids 1-149

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MAPKAP1RBD

Plasmid

#120710

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B WT

Plasmid

#171770

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B WT

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

D433N

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B D433N

Plasmid

#171823

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSIV-D3psi/delta env/delta Vif/delta Vpr

Plasmid

#132928

Purpose

Production of SIV-VLPs containing Vpx protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

Gag, pol, and vpx

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI934

Plasmid

#216637

Purpose

Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHR CD19-FcGamma CAR

Plasmid

#113014

Purpose

for production of lentivirus encoding the CD19 CAR-Pfcgamma

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular GFP (CD19 Human, Mouse, Synthetic)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHR CD19 tandem CAR

Plasmid

#113019

Purpose

for lentivirus production of CD19 tandem CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Human, Mouse, Synthetic)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Rac1-2G

Plasmid

#66111

Purpose

Second generation Rac1 FRET biosensor for lentivirus production

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pNTI827

Plasmid

#216635

Purpose

Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID-ERM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI823

Plasmid

#216632

Purpose

Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID (split site variant)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI828

Plasmid

#216636

Purpose

cpTurboID-ERM (V5) ER membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID-ERM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI935

Plasmid

#216638

Purpose

cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-BclXL-Blasticidin

Plasmid

#120718

Purpose

Expresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Bl2-like protein 1, isoform Bcl-X(L)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

33 c-terminal amino acids

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MAPKAP1RBD/Triple Mutant

Plasmid

#120711

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353, K307A, K310A, R312L

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-MAPKAP1Del-FHH-IRES-Puro

Plasmid

#120706

Purpose

Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 Isoform 1

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Deleted amino acids 193-522

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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miR5 Mm ATP13A2

Plasmid

#171822

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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miR3 Mm ATP13A2

Plasmid

#171771

Purpose

transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

ATP13A2 (Atp13a2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge