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Showing: 141 - 160 of 454 results

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Clathrin-mhYFP

Plasmid

#186527

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Clathrin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-rpb1-E1103G

Plasmid

#10989

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1103G (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 3308 A to G in the RPB1 ORF resulting in…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
3185 pBS Bax cDNA

Plasmid

#8754

Depositor

Stanley Korsmeyer

Article

Knudson et al Science 1995 Oct 6;270(5233):96-9.

Insert

Bax (Bax Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pEasy Flox

Plasmid

#11725

Depositor

Klaus Rajewsky

Article

Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

Insert

pEasyFlox

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
rbcLS-pMAL-2px

Plasmid

#41621

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

rbcL/rbcS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-rpb1-N488D

Plasmid

#10988

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-N488D (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 1462 A to G in the RPB1 ORF resulting in…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
3177 pSFFV-neo HA Bax alpha

Plasmid

#8810

Depositor

Stanley Korsmeyer

Article

Knudson et al Science 1995 Oct 6;270(5233):96-9.

Insert

Bax alpha (Bax Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C67Y

Plasmid

#10982

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C67Y (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 200 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C70Y

Plasmid

#10983

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C70Y (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 209 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-H80Y

Plasmid

#10984

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-H80Y (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 238 C to T in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only