-
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2
Plasmid#203605PurposeReporter plasmid carrying PHLPP2 3'UTRDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-27bT
Plasmid#203600PurposeReporter plasmid carrying miR-27b target sequenceDepositorInsertcomplementary sequence to miR27b-3p (MIR27B Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-A'Q-mito
Plasmid#158766PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'Q-mito
Plasmid#158767PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
-
pT3-EF1a-Bmi1
Plasmid#31783DepositorInsertBmi1 (BMI1 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pK170.AAV-TRE-Cre-WPRE (Supernova)
Plasmid#85040PurposeFor AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.DepositorInsertnlsCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-YY1
Plasmid#154943PurposeKnocking down of human YY1 through shRNA and amiRNA targeting YY1DepositorInsertshRNA and amiRNA against YY1 (YY1 Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(AI)
Plasmid#111500PurposeExpresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertYFP-SMASh(AI)
UseTagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(TI)
Plasmid#111501PurposeExpresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].DepositorInsertYFP-SMASh(TI)
UseTagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-StaPLd-Casp9
Plasmid#111511PurposeExpresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertStaPLd-Casp9
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NS3/4A
Plasmid#186884PurposeDoxycycline-dependent expression of NS3/4A protease gene in mammalian cells by retroviral transductionDepositorInsertHCV NS3/4A protease
UseRetroviralTagsExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MbPylRS(DiZPK)
Plasmid#91706Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionMammalianMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hPTGS2-2flag
Plasmid#102498PurposeMammalian expression of PTGS2DepositorInsertPTGS2 (PTGS2 Human)
UseTagsFlag-FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV FLAG Rev-Erb alpha
Plasmid#22745DepositorInsertRev Erb alpha (NR1D1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack: shRev-Erbalpha
Plasmid#22749DepositorInsertshRev-Erbalpha (Nr1d1 Mouse)
UseAdenoviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-FOXO3 S574A
Plasmid#52426Purposemammalian expression of FOX03 with mutant JNK phosphorylation siteDepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationS574A, mutant JNK phosphorylation sitePromoterAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-FOXO3 R248K R250K
Plasmid#52427Purposemammalian expression of FOXO3 methylation resistant mutantDepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationR248K R250K, mutant methylation sitesPromoterAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only