Addgene

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pBW2272_pCAG-PV1-FlpO-N27-L1-FRB-NLS-BGHpA

Plasmid

#108755

Purpose

Expresses N-terminus to aa27 of Flp recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa27

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2273_pCAG-PV1-FKBP-L1-FlpO-28C-NLS-BGHpA

Plasmid

#108756

Purpose

Expresses aa28 to C-terminus of Flp recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2276_pCAG-PV1-FlpO-N168-L1-FRB-NLS-BGHpA

Plasmid

#108757

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2277_pCAG-PV1-FKBP-L1-FlpO-169C-NLS-BGHpA

Plasmid

#108758

Purpose

Expresses aa169 to C-terminus of Flp recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa169 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2280_pCAG-PV1-FlpO-N374-L1-FRB-NLS-BGHpA

Plasmid

#108759

Purpose

Expresses N-terminus to aa374 of Flp recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa374

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2281_pCAG-PV1-FKBP-L1-FlpO-375C-NLS-BGHpA

Plasmid

#108760

Purpose

Expresses aa375 to C-terminus of Flp recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa375 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2284_pCAG-PV1-FlpO-N396-L1-FRB-NLS-BGHpA

Plasmid

#108761

Purpose

Expresses N-terminus to aa396 of Flp recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa396

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2285_pCAG-PV1-FKBP-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108762

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA

Plasmid

#108749

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpA

Plasmid

#108750

Purpose

Expresses aa169 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa169 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpA

Plasmid

#108751

Purpose

Expresses N-terminus to aa374 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa374

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA

Plasmid

#108752

Purpose

Expresses aa375 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa375 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

Plasmid

#68347

Purpose

AAV plasmid expressing the FlpO recombinase. with empty gRNA

Depositor

Jannik Elverløv-Jakobsen

Insert

FlPO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

Plasmid

#108833

Purpose

Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS and GAI

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

Plasmid

#68405

Purpose

The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

Depositor

Jannik Elverløv-Jakobsen

Inserts

3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO

Use

CRISPR

Tags

mTurquoise2 (BFP)

Expression

Mammalian

Mutation

Promoter

Synthetic Probasin ARRx2 and U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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AiP1635 - pAAV-AiE2116m-minBG-DIO-FlpO-WPRE-BGHpA

Plasmid

#230680

Purpose

AiE2116m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14573: pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA (Alias: CN4573)

Plasmid

#230871

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.

Insert

FlpO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14826: pAAV-AiE0441h_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4826)

Plasmid

#214863

Purpose

Expression of FlpO in striatal medium spiny neurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14201: pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4201)

Plasmid

#214855

Purpose

Expression of FlpO in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)

Plasmid

#159222

Purpose

Can be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpO

Depositor

Rui Benedito

Article

Luo et al Nature. 2021 Jan;589(7842):437-441. doi:

Insert

Apln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln

Use

Tags

Expression

Mammalian

Mutation

Promoter

Apln

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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