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Plasmid#157669PurposeRetroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.DepositorInsertHistone H2B
UseRetroviralTagsFast-FTExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-Flag-FANCD2
Plasmid#134904Purposecodon optimized expression and purification of human FANCD2 in insect cells using baculovirusDepositorInsertFANCD2 (FANCD2 Human)
UseTagsFlagExpressionInsectMutationfully synthetic, codon optimized for insect expre…PromoterpolyhedronAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 Flag BRG1
Plasmid#1957DepositorInsertBRG1 (SMARCA4 Human)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF16
Plasmid#52642Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF16
UseTagsExpressionMammalianMutationD431N/D433N/D435N/E442A mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG671 mito-pFAST
Plasmid#172867PurposeExpresses mitochondrial targeting domain-pFAST in mammalian cellsDepositorInsertmito-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF19
Plasmid#52644Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF19
UseTagsExpressionMammalianMutationT328D/T330D mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG657 H2B-pFAST
Plasmid#172862PurposeExpresses pFAST fused to zebrafish histone H2B in mammalian cellsDepositorInsertH2B-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG876 pDisplay-pFAST
Plasmid#172868Purposeexpresses pFAST at the surface of mammalian cellsDepositorInsertpFAST-PDGFR
UseTagsIgK leader sequence (N terminal on insert) and My…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Shp2)
Plasmid#177899PurposeBaculo expression of His10 tag fused to human Shp2DepositorInsertShp2 (PTPN11 Human)
UseTags10xHis-TEV-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorInsertLis1 (PAFAH1B1 Human)
UseTags8x His, Linker-TEV-linker_TEV, and ZZExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG506-mito-frFAST
Plasmid#160057PurposeExpresses mito-frFAST in mammalian cellsDepositorInsertMito-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(fast)
Plasmid#107690PurposeN-terminal half of π-EB1 with fast LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain (I427V, H519L…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG384 bFos-CFAST11
Plasmid#130815PurposeExpresses bFos-CFAST11 in mammalian cellsDepositorInsertbFos-CFAST11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Fast-FT
Plasmid#31916DepositorInsertFast-FT
UseTagsHisExpressionBacterialMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsHis6ExpressionInsectMutationnonePromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG665 MAP4-pFAST
Plasmid#172865PurposeExpresses MAP4-pFAST in mammalian cells (microtubule labeling)DepositorInsertMAP4-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1/MBP-His
Plasmid#38008DepositorInsertMBP (Maltose binding protein)
UseBaculovirus expressionTags6xHISExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG463-FKBP-redNFAST
Plasmid#160364PurposeExpresses FKBP-redNFAST in mammalian cellsDepositorInsertFKBP-redNFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9L
Plasmid#166788PurposeInsect cell expression vector for DPP9L (protein purification)DepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only