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Plasmid#228710PurposeMammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibodyDepositorArticleInsertC143 LC N28R
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG2a_M18
Plasmid#105930PurposeExpression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertIgG2b heavy chain (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Golgin-84-TEV #709#243
Plasmid#42108DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsGFPExpressionMammalianMutationinserted ENLYFQG b/w 300 and 301PromoterCMVAvailable sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_F(ab')2
Plasmid#105860PurposeExpression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertIgG3 F(ab')2 (Ighg3 Mouse)
UseTagsExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_Lys322Arg
Plasmid#105862PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationLys322Arg in constant region of IgG1 heavy chainPromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAF124
Plasmid#222372PurposeRecombinant human IgG1 antibody (3D5) that binds to 6xHis at the C-terminus of proteinsDepositorInsertIGHG
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJD1
Plasmid#222374PurposeRecombinant human IgG1 antibody (1F8) that binds to Tasmanian devil PDL1DepositorInsertIGHG
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pComb3
Plasmid#63888PurposeFor phage display of antibody libraries by fusion to modified geneIII.DepositorTypeEmpty backboneUsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pComb8
Plasmid#63889PurposeFor phage display of antibody libraries by fusion to modified geneVIII.DepositorTypeEmpty backboneUsePhage displayTagsgeneVIIIExpressionBacterialMutationPromoterlacZAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF125
Plasmid#222373PurposeRecombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.DepositorInsertIGHG (partial)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF201
Plasmid#222376PurposeRecombinant Tasmanian devil IgG antibody (1F8) that binds to Tasmanian devil PDL1DepositorInsertIGHG
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF200
Plasmid#222375PurposeRecombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteinsDepositorInsertIGHG
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_Arg322Lys
Plasmid#105849PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationArg322Lys in constant region of IgG1 heavy chainPromoterhEF1-HTLVAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-LC122-TAG
Plasmid#226839PurposeContains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationamino acid 122 of light chain mutated to TAGPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-HC121-TAG
Plasmid#226836PurposeContains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationamino acid 121 of heavy chain mutated to TAGPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-HC197-TAG
Plasmid#226838PurposeContains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationamino acid 197 of heavy chain mutated to TAGPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2charge
Plasmid#105858PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG3, muatations: Hi…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only