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pAc5.1B-lambdaN-HA-Dm4E-T-FWK41AAA_T

Plasmid

#147611

Purpose

Insect Expression of Dm4E-T-FWK41AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-FWK41AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-NRE90AAA_T

Plasmid

#147577

Purpose

Insect Expression of Dm4E-T-NRE90AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-NRE90AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T-NRE90AAA_T

Plasmid

#147582

Purpose

Insect Expression of Dm4E-T-NRE90AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-NRE90AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148410

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148429

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTS2415-Tier1-PPGK-1xEAAAK

Plasmid

#169482

Purpose

Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven EAAAK-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPD387 VPR-ZF43(AAAR)

Plasmid

#138825

Purpose

Constitutive expression of VPR-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD386 VP64-ZF43(AAAR)

Plasmid

#138821

Purpose

Constitutive expression of VP64-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD384 VP64-ZF43(RAAA)

Plasmid

#138820

Purpose

Constitutive expression of VP64-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD112 VP16-ZF43(AAAR)

Plasmid

#138817

Purpose

Constitutive expression of VP16-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pPD109 VP16-ZF43(RAAA)

Plasmid

#138814

Purpose

Constitutive expression of VP16-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DED/AAA USP7

Plasmid

#131253

Purpose

Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Contains point mutations that convert USP7 aspart…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG

Plasmid

#127306

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90

Plasmid

#26192

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Jul 29. ():.

Insert

23S ribosomal RNA fragment (modified) with duplex H90

Use

Tags

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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23SrRNA.RAV12(wt).2508-2580.G2530AAAC

Plasmid

#26190

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Jul 29. ():.

Insert

23S ribosomal RNA fragment (modified)

Use

Tags

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U

Plasmid

#26191

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Jul 29. ():.

Insert

23S ribosomal RNA fragment (modified) with G2553U mutation

Use

Tags

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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T4 Lysozyme S44-[AAA] L46A WT*

Plasmid

#18319

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Use

Tags

Expression

Bacterial

Mutation

S44-[AAA] L46A WT*

Promoter

Available since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only
pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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