Showing: 141 - 160 of 204 results
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Plasmid#107072PurposeHost vector to construct a YPer or CyPet fusion proteinDepositorInsertYPet-CyPet fusion protein
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
Plk1 FRET sensor c-jun substrate
Plasmid#45203DepositorInsertPlk1 FRET sensor with c-jun substrate
UseTagsCyPET, YPETExpressionMammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
N-term Hec1 Plk1 FRET sensor c-jun substrate
Plasmid#45204DepositorInsertHec1-targeted Plk1 FRET sensor
UseTagsCyPET, YPETExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaNAR2
Plasmid#64728PurposeFRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertCaNAR2
UseTagsCerulean3 and YPetExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-YFP
Plasmid#90104PurposeYFP control expression plasmidDepositorInsertYFP
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-CaNAR2
Plasmid#64729PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertCaNAR2-NES
UseTagsCerulean3, Nuclear export signal (NES), and YPetExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-CaNAR2
Plasmid#64730PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertLyn-CaNAR2
UseTagsCerulean3, N-terminal targeting sequence from Lyn…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-E2
Plasmid#175243PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to YpetDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
PI(DM)-Rac1(WT)dc1.FLARE
Plasmid#87589PurposePI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PI(LM)-Rac1(WT)dc1.FLARE
Plasmid#87590PurposePI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
untargeted Aurora B FRET sensor Kif2 substrate
Plasmid#45215DepositorInsertuntargeted Aurora B FRET sensor
UseTagsCyPET, YPETExpressionMammalianMutationPromotert7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-CaNAR2
Plasmid#64732PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNAR2
UseTagsCerulean3, N-terminal 27 amino acids from CYP450,…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-4146NES(ROCK)
Plasmid#162485PurposeFor generating transgenic mice expressing ROCK biosensor based on FRET.DepositorInsertROCK biosensor based on FRET.
UseTagsYPet and ECFPExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-HCA_sensor
Plasmid#127181PurposeBacterial expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
UseTagsHisTag and StrepTagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-HCA_sensor
Plasmid#127182PurposeEukaryotic expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
UseTagsHisTag and StrepTagExpressionMammalianMutationPromoterCMV-TetO2AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-p52-HDM2_sensor
Plasmid#127184PurposeEukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interactionDepositorInsertp53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
UseTagsHisTag and StrepTagExpressionMammalianMutationPromoterCMV-TetO2AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR
Plasmid#64927PurposeFRET-based biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR(T/A)
Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 141 - 160 of 204 results