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Plasmid#38251DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only
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pET22b-CPDSacI
Plasmid#38255DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HA-CPDSalI
Plasmid#38254DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET22b-HA-CPDSalI
Plasmid#38253DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQdCas9-sgempty
Plasmid#159523PurposeExpression of dCas9 driven by lux cassette from Vibrio fischeriDepositorInsertquorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-GFP-CPDSalI
Plasmid#38257DepositorInserteGFP
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pEGFPN3 (Clonetech)PromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNPK-NLS-Vika
Plasmid#79969Purposeexpresses human codon-optimized Vika-recombinase in mammalian cellsDepositorInserthuman codon-optimized Vika sequence, NLS tagged
UseTagsNLSExpressionMammalianMutationPromoterPGKAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-vox-EGFP
Plasmid#79970Purposemammalian GFP reporter plasmid, target site for Vika recombinaseDepositorInsert2 vox-sites flanking neo in frame with EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC0.062
Plasmid#119651PurposeLevel 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.DepositorInsertBba_B0011
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24_BD3179
Plasmid#73142PurposeExpresses in E.coli B. bacteriovorus Bd3179DepositorInsertBd3179
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMO9075
Plasmid#117481Purposeempty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecRDepositorInsertAph(3')-IIa promoter
UseTagsExpressionBacterialMutationPromoterAph(3')-IIa promoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas7/pHMGWA
Plasmid#81184PurposeExpresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas5c/pHMGWA
Plasmid#81182PurposeExpresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas5c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas8c/pHMGWA
Plasmid#81183PurposeExpresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas8c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only