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pA-01

Plasmid

#166923

Purpose

LacI expression cassette (A)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

LacI expression cassette - Block A- Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcR295

Plasmid

#17678

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Lys 295 changed to Arg.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pSTC0

Plasmid

#39240

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ11m

Plasmid

#39245

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcR295/527

Plasmid

#17679

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Lys 295 changed to Arg. Tyr 527 changed to Phe.

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pVB004

Plasmid

#127188

Purpose

Tunable NOT gate without RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate and Reporter without RiboJ Insulator

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRAJ11

Plasmid

#39244

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12m

Plasmid

#39247

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21m

Plasmid

#39250

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSTC0r1

Plasmid

#39242

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

pLlacO-1+BBa_B0034+GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcSrc416/527

Plasmid

#17677

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y416F Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Tyr 416 changed to Phe . Tyr 527 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Use

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_pScaC(50-159)

Plasmid

#228836

Purpose

E. coli expression of a truncated ScaC passenger domain (aa 50-159)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Use

Tags

Strep

Expression

Bacterial

Mutation

aa 50-159

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Use

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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50_p5E_UBI_loxTagBFP

Plasmid

#172518

Purpose

p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision

Depositor

Jean Giacomotto

Article

Guo et al bioRxiv 2024.02.22.581357

Insert

ubiquitin promoter, TagBFP, loxP sites (inversion)

Use

Tags

Expression

Mutation

Promoter

Ubiquitin

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSTAR2

Plasmid

#166940

Purpose

STAR-based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Small transcription activating RNAs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSTAR

Plasmid

#166939

Purpose

STAR-based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Small Transcription activating RNA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge