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Plasmid#116099PurposeExpresses a combination of proteases and their reporterDepositorInsertCasp-rastvTEVca-teTVMVd1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-TO-Casp-rastvTEV-teTVMV1
Plasmid#116097PurposeExpresses a combination of proteases and their reporterDepositorInsertCasp-rastvTEV-teTVMV1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-TO-Casp-rastvTEV-teTVMV2
Plasmid#116098PurposeExpresses a combination of proteases and their reporterDepositorInsertCasp-rastvTEV-teTVMV2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)
Plasmid#44083DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)
Plasmid#44095DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)
Plasmid#44071DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)
Plasmid#44073DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
His6TEV-UbG76C
Plasmid#75299PurposeBacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV proteaseDepositorInserthuman Ubiquitin mutant G76 to C
UseTagsHisExpressionBacterialMutationchanged Glycine 76 to CystinePromoterT7Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-TEV-Nsp14
Plasmid#154954PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsert6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22-Nsp10-TEV-6His
Plasmid#154957PurposeExpresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp10-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-Nsp14-TEV-6His
Plasmid#154952PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp14-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-Strep-TEV-Nsp14
Plasmid#154955PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertStrep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
UseTagsStrep-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-Nsp14-TEV-Strep
Plasmid#154953PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertNsp14-TEV-Strep (ORF1ab SARS-CoV-2)
UseTagsTEV-StrepExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22-Strep-TEV-Nsp10
Plasmid#154958PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertStrep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
UseTagsStrep-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
Plasmid#44094DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)
Plasmid#44081DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)
Plasmid#44082DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)
Plasmid#44070DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)
Plasmid#44093DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)
Plasmid#44069DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only