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Plasmid#135925PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mAzurite
UseTransposonTagsmAzuriteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF197
Plasmid#135930PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).DepositorInsertCTLA4 fused to IgG Fc and to mCherry
UseTransposonTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF164
Plasmid#135928PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mOrange
UseTransposonTagsmOrangeExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF139
Plasmid#135924PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mTagBFP
UseTransposonTagsmTagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF92
Plasmid#135929PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).DepositorInsertmCherry fused to TNFSF9
UseTransposonTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF138
Plasmid#135923PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mCitrine
UseTransposonTagsmCitrineExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF161
Plasmid#135926PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mCerulean3
UseTransposonTagsmCerulean3ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF163
Plasmid#135927PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mNeptune2
UseTransposonTagsmNeptune2ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.2
Plasmid#201404PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet-Seq1.1
Plasmid#201401PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/Col1a1/GFP4_Seq 1.1
Plasmid#201400PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-Col1a1/GFP4_Seq1.2
Plasmid#201403PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmGamillus
Plasmid#182971PurposeStably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmGamillus
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2/GD-IRES-GFP-CTNNB1
Plasmid#192868PurposeCarries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.DepositorInsertsluc+
EGFP
CTNNB1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Plasmid#68345PurposeA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expressionDepositorInsertspuromycin
hCas9
UseCRISPR; Flp/frtTagslinked to red flouresent protein gene mKateII and…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA3 (pLP305)
Plasmid#196992PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2-hUbC-dCas9-KRAB-T2a-Puro
Plasmid#225999PurposeTo establish stable dCas9-KRAB knock-in lineDepositorInserthumanized dCas9-KRAB T2A Puro
UseSleeping beauty transposonTagsExpressionMammalianMutationD10A and H840APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SB
Plasmid#177936PurposeExpresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits