Addgene

We narrowed to 4,139 results for: Pet2;

Showing: 141 - 160 of 4139 results

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET24a_UDP_Xase_E113

Plasmid

#73159

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

change glutamate 113 to alanine

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_1_120

Plasmid

#65730

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1594

Plasmid

#11413

Depositor

Sung-Hou Kim

Article

Wang et al Structure. 2001 Jan 10. 9(1):65-71.

Insert

phosphoserine phosphatase (MJ_1594 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_20_101

Plasmid

#65733

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-101

Promoter

T7

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0697

Plasmid

#11411

Depositor

Sung-Hou Kim

Article

Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

Insert

fibrillarin homolog (MJ_0697 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

M222T

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1247

Plasmid

#11414

Depositor

Sung-Hou Kim

Article

Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

Insert

3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)

Use

Tags

T7-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0882

Plasmid

#11417

Depositor

Sung-Hou Kim

Article

Huang et al J Struct Funct Genomics. 2002 . 2(3):12

Insert

methyltransferase (MJ_0882 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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CtkA_full_pET21a

Plasmid

#27428

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_300_pET21a

Plasmid

#27429

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

residues 301-325 are truncated

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q_pET21a

Plasmid

#27430

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D155Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D179Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0307

Plasmid

#11415

Depositor

Sung-Hou Kim

Article

Cave et al Protein Sci. 2001 Feb . 10(2):384-96.

Insert

disulfide oxidoreductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pET21a.TM1363

Plasmid

#11420

Depositor

Sung-Hou Kim

Article

Shin et al J Mol Biol. 2004 Jul 30. 341(1):227-39.

Insert

peptide release factor I (prfA Thermotoga maritima)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pET28a_HCK

Plasmid

#224239

Purpose

Expression of human HCK Kinase in E. coli

Depositor

Andrew Mesecar

Insert

hck (HCK Human)

Use

Tags

6xHis and 6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-M2

Plasmid

#225735

Purpose

To express M2 (219-399) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 219-399

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a_PTK2b

Plasmid

#224237

Purpose

Expression of human PTK2b Kinase in E. coli

Depositor

Andrew Mesecar

Insert

ptk2b (PTK2B Human)

Use

Tags

6xHis and 6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-M1

Plasmid

#225734

Purpose

To express M1 (13-193) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 13-193

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET27b+R

Plasmid

#188245

Purpose

Empty backbone vector for protein expression in Escherichia coli and his-tag purification

Depositor

Yiping Qi

Article

Fang et al Methods Mol Biol. 2023;2653:153-171. doi

Type

Empty backbone

Use

Tags

6xHis tag and TEV site

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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