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mTFP1-Lysosomes-20

Plasmid

#55495

Purpose

Localization: Lysosome Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LAMP1

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mRuby2-Lysosomes-20

Plasmid

#55902

Purpose

Localization: Lysosome Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Use

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Clover-Lysosomes-20

Plasmid

#56528

Purpose

Localization: Lysosome Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-Lysosomes-20

Plasmid

#56228

Purpose

Localization: Lysosome Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LAMP1

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Lysosomes-20

Plasmid

#56166

Purpose

Localization: Lysosome Membrane, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

LAMP1

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pTol2-autophagosome-lysosome reporters

Plasmid

#196135

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

autophagosome-lysosome reporters

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-F-actin-microtubule plus ends reporters

Plasmid

#196134

Purpose

zebrafish expression

Depositor

Reinhard Köster

Insert

F-actin-microtubule plus ends reporters

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge