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Plasmid#215295PurposeExpresses sfGFP under the Anderson J23107 promoter with a BBR1 backbone.DepositorInsertsfGFP
UseTagsHisExpressionBacterialMutationPromoterJ23107Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC12
Plasmid#197406PurposeHoney bee gut symbiont broad-host range vector with constitutive GFP, ampR and pBBR1 repliconDepositorInsertpBBR1 replicon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC23
Plasmid#197409PurposeHoney bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pBBR1 repliconDepositorInsertpBBR1 replicon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC04
Plasmid#197400PurposeHoney bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pBBR1 repliconDepositorInsertpBBR1 replicon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSJ182
Plasmid#159723PurposeExpress Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID4 (VID24 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMVV098
Plasmid#215304PurposeExpresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPPhIFAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 wt
Plasmid#80876Purposemammalian expression of ALK5 WTDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVV205
Plasmid#215342PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backboneDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Tc
Plasmid#222336Purposeflp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3DepositorUseTagsExpressionMutationPromotercl, flp, and sacBAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVV102
Plasmid#215308PurposeExpresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPTacAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV103
Plasmid#215309PurposeExpresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPtetAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV104
Plasmid#215310PurposeExpresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPAraBADAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1009 (pSL9)
Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP4518
Plasmid#107778PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pBBR1-mcs5
UseTagspLacZ-EYFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP4516
Plasmid#107775PurposeExpression of ECFPDepositorInsertpLacZ-ECFP in pBBR1-mcs5
UseTagspLacZ-ECFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only