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Plasmid#72409PurposeProduces Acetobacter aceti 1023 Est10DepositorInsertferuloyl esterase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK378
Plasmid#72412PurposeProduces Acetobacter aceti 1023 Est07DepositorInsert4-hydroxybenzoyl-CoA thioesterase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK639
Plasmid#72398PurposeProduces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)DepositorInsertamidophosphoribosyltransferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK436
Plasmid#72477PurposeProduces Thermoplasma acidophilum citrate synthaseDepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK616
Plasmid#72460PurposeProduces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK531
Plasmid#72512PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK645
Plasmid#71700PurposeProduces E. coli thioredoxin (EcTrxA)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK266
Plasmid#73326PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tagDepositorInsertviolacein biosynthesis protein VioD
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK409
Plasmid#72423PurposeProduces Acetobacter aceti 1023 Est14, C-terminal His6 tagDepositorInsertarylesterase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK405
Plasmid#72418PurposeProduces Acetobacter aceti 1023 Est13, N-terminal His6 tagDepositorInsertarylesterase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK403
Plasmid#72416PurposeProduces Acetobacter aceti 1023 Est07, N-terminal His6 tagDepositorInsertarylesterase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK690
Plasmid#72380PurposeProduces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)DepositorInsertglutaredoxin-4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK365
Plasmid#72404PurposeProduces Acetobacter aceti 1023 Est01DepositorInsertacyl-CoA thioester hydrolase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK241
Plasmid#71693PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Tro404 to PhePromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK699
Plasmid#71691PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Asp371 to AsnPromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK708
Plasmid#71692PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Trp400 to PhePromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK195
Plasmid#73321PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tagDepositorInsertviolacein biosynthesis protein VioC
UseTagsHis6ExpressionBacterialMutationcontains silent mutation in Val428PromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK521
Plasmid#72440PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseUnspecifiedTagsExpressionMutationsynthetic gene with ~40 silent substitutionsPromotern/aAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK536
Plasmid#72444PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseTagsExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK547
Plasmid#72449PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationVal319 to PhePromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK550
Plasmid#72450PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationinserted Val2PromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK539
Plasmid#72515PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK549
Plasmid#72520PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK555
Plasmid#72521PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK577
Plasmid#72528PurposeEncodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseGene synthesisTagsExpressionMutationPromotern/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK633
Plasmid#72397PurposeProduces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)DepositorInsertphosphoribosylaminoimidazole synthetase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK615
Plasmid#71699PurposeProduces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)DepositorInsertthioredoxin
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK647
Plasmid#71701PurposeProduces E. coli thioredoxin reductase (EcTrxB)DepositorInsertthioredoxin reductase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK408
Plasmid#72422PurposeProduces Acetobacter aceti 1023 Est10, C-terminal His5 tagDepositorInsertferuloyl esterase
UseTagsHis5ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK367
Plasmid#72406PurposeProduces Acetobacter aceti 1023 Est06DepositorInsertlipase
UseTagsExpressionBacterialMutationlacks Met1 and Thr2 in annotated (v1) sequencePromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK402
Plasmid#72415PurposeProduces Acetobacter aceti 1023 Est03, N-terminal His6 tagDepositorInsert4-hydroxybenzoyl-CoA thioesterase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK580
Plasmid#71694PurposeProduces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)DepositorInsertthioredoxin
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK532
Plasmid#72513PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK544
Plasmid#72517PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E182 Hs.PIK3R6-nostop
Plasmid#70466PurposeGateway ORF clone of human PIK3R6 [NM_001010855.3] without stop codon (for C-terminal fusions)DepositorInsertPIK3R6 (PIK3R6 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK546
Plasmid#72518PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E181 Hs.PIK3R6
Plasmid#70465PurposeGateway ORF clone of human PIK3R6 [NM_001010855.3] with stop codon (for native or N-terminal fusions)DepositorInsertPIK3R6 (PIK3R6 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK567
Plasmid#72522PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK561
Plasmid#72283PurposeProduces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationchanges cysteine-35 to serinePromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK523
Plasmid#72442PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)DepositorInserthypothetical protein
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK126
Plasmid#73337PurposeEncodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 328-685PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK323
Plasmid#73495PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK366
Plasmid#72405PurposeProduces Acetobacter aceti 1023 Est02DepositorInsertpeptidase S9
UseTagsExpressionBacterialMutationadditional N-terminal MVKNGS sequence, alternate …PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK377
Plasmid#72411PurposeProduces Acetobacter aceti 1023 Est05DepositorInserthypothetical protein
UseTagsExpressionBacterialMutationadditional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK322
Plasmid#73494PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromotern/aAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK614
Plasmid#72387PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 4 silent mutations in codons 9-11 and 42…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK621
Plasmid#72388PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK592
Plasmid#72386PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK684
Plasmid#71638PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to valinePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only