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Showing: 351 - 400 of 404 results

  1. pCLV3Cas-LT1

    Plasmid
    #210549
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    Use
    Tags
    Expression
    Plant
    Mutation
    2bp insertion after AAVS1 target sequence that di…
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210549-plasmid-map-sequence-id-426236

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  2. LPUtopia-8

    Plasmid
    #224781
    Purpose
    Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Inserts
    puromycin N-acetyltransferase
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224781-plasmid-map-sequence-id-445949

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  4. pCLV3Cas-LT2

    Plasmid
    #210550
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
    Use
    Tags
    Expression
    Plant
    Mutation
    1bp insertion after AAVS1 target sequence that di…
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210550-plasmid-map-sequence-id-426233

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  14. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  37. MTK0_051

    Plasmid
    #123948
    Purpose
    Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hAAVS1 landing pad destination vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123948-plasmid-map-sequence-id-250127

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  38. MTK0_057

    Plasmid
    #123950
    Purpose
    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    BxBI LP on hAAVS1 (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    123950-plasmid-map-sequence-id-271459

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  41. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

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  50. ipYUC2Cas-LT1

    Plasmid
    #210559
    Purpose
    Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    210559-plasmid-map-sequence-id-426227

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Showing: 351 - 400 of 404 results