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Plasmid#220835PurposeTALEcharm expression in mammalian cellsDepositorInsertTALEcharm Kv2
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB398_ZFcharm_Prnp_SMM
Plasmid#220845PurposeAAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorArticleInsertDDX5ΔPrD(1-535)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP
Plasmid#166151PurposeExpression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coliDepositorArticleInsertDDX5ΔIDR(1-483)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔIDR(1-483)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB352_ZFcharm_Kv1_split-inteins
Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB304_ZFcharm_Kv1_Prnp_SCR
Plasmid#220842PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB301_ZFcharm_Kv1_Prnp_SPM
Plasmid#220840PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1204-pOCC120-FUS_opt(NheI)
Plasmid#221892PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTagsMBP-PreScission and mGFP-PreScission-6xHisExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221893PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
UseTags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB400_ZFcharm_Prnp_DPM
Plasmid#220847PurposeAAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB399_ZFcharm_Prnp_SPM
Plasmid#220846PurposeAAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB340_ZFcharm_Kv1_Prnp_DPM
Plasmid#220843PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD deltahexa
Plasmid#169714PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deletedDepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
WT A1-LCD +NLS
Plasmid#178658PurposeBacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)DepositorInsertWT A1-LCD +NLS (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP
Plasmid#221891PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1263-018_Jie_SFPQ_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221895PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertSFPQ (SFPQ Human)
UseTags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB303_ZFcharm_Kv1_Prnp_SMM
Plasmid#220841PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; single mismatch self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-10R
Plasmid#178664PurposeBacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues removed (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-10R (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationten Arg residues removedPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+8D
Plasmid#178674PurposeBacterial expression of N-terminally 6His tagged A1-LCD with eight Asp residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+8D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationeight Asp residues addedPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-9F+3Y
Plasmid#178663PurposeBacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-9F+3Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationnine Phe residues removed, three Tyr addedPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only