These results may also be relevant.
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His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSDM052
Plasmid#194341PurposepUC mini-Tn7 vector expressing LseCas13aDepositorInsertCas13a(Lse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4
Plasmid#135460PurposeMammalian expression of uFLARE TF componentDepositorInsertCMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
UseTagsHA, flag,ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1
Plasmid#39349DepositorUseYeast genomic targetingTagskanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-Purg1
Plasmid#39350DepositorUseYeast genomic targetingTagsnatMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA
Plasmid#135462PurposeNeuronal expression of uFLARE TF componentDepositorInsertNRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
UseAAVTagsHA, flag, VP16ExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Syn:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135463PurposeNeuronal expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseAAVTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5
Plasmid#135456PurposeBacterial expression of uTEV2Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, T30A/S153N mut…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ch-gelsolN
Plasmid#37262DepositorInsertgelsolin (GSN Human)
UseTagsmCherryExpressionMammalianMutationdeleted amino acids 380-731PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-3HA
Plasmid#39351DepositorUseYeast genomic targetingTags3xHA and kanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceJan. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-GFP
Plasmid#39353DepositorUseYeast genomic targetingTagsGFP and kanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Nedd4.2F823A
Plasmid#165104Purposebacterial expression of a GST fusion of Human Nedd4.2d F823ADepositorInsertNedd4.2d F823A (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationF823A; mutation for destabilization of trimer fo…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-GST
Plasmid#39352DepositorUseYeast genomic targetingTagsGST and kanMX6 module - reverse orientationExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-626_pGEX6P1
Plasmid#209790PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
UseTagsGSTExpressionBacterialMutationaa 544-626 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2F823D
Plasmid#165105Purposebacterial expression of a GST fusion of Human Nedd4.2d F823DDepositorInsertNedd4.2d F823D (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expression; F823D; …PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-MKLP1
Plasmid#70154Purposemammalian expression of MKLP1 with an mCherry fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
UseTagsBFP, flagExpressionYeastMutationPromoterTDH3Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
