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Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9-AIDx
Plasmid#135343PurposeExpression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorInsertnSpyCas9-hAIDx
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpyTag-GlpG
Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
UseTags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCsn2
Plasmid#113251PurposeE. coli expression vector for S. pyogenes Csn2-MBP-HisDepositorInsertS. pyogenes Csn2
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG-B-SpyTag-C
Plasmid#87373PurposeAn ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assemblyDepositorInsertSpyTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCas1
Plasmid#113248PurposeE. coli expression vector for His-MBP-S. pyogenes Cas1DepositorInsertS. pyogenes Cas1
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCas2
Plasmid#113249PurposeE. coli expression vector for His-MBP-S. pyogenes Cas2DepositorInsertS. pyogenes Cas2
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG-B-mSpyCatcher-C
Plasmid#87374PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assemblyDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-SpyTag
Plasmid#67176PurposeCRISPaint universal donor for SpyTag fusion taggingDepositorInsertSpyTag
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only