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Plasmid#16370DepositorInsertOrai3 (ORAI3 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P23
Plasmid#120034PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorInsertOCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD63-pHuji
Plasmid#130902PurposeExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD63-pHuji (CD63 synthetic, Human)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Gln-36 and Leu-37 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TfR-OVA
Plasmid#64600PurposeExpression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentationDepositorInsertOvalbumin
UseTagsTransmembrane domain of Transferrin Receptor (TfR…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B
Plasmid#106309PurposeThe plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.DepositorInsert6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
UseTags6xflag-3xHaloTag(MtoL)ExpressionMammalianMutationPromoterEF1Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-NES
Plasmid#36206PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#172196PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sitesDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2
Plasmid#40260PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertmRuby2
UseTagsHis6 and XpressExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only