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pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-LacZ-BbsI
Plasmid#114671PurposeFor one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expressionDepositorInsertLacZ
UseTagsExpressionMammalianMutationPromoterLac promoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
p404MET3
Plasmid#17419DepositorInsertMET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pQC V5 MCS IRES Puro
Plasmid#110342Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 hygro sgUCK1
Plasmid#211524PurposeDeletes UCK1DepositorInsertsgUCK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
371-C
Plasmid#97029PurposeHis6-MBP-N10-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, MBP domain, N10 linker, and TEV cleavag…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromotertetR/pLtetOAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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p406MET3
Plasmid#17436DepositorInsertMET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmRFPmars
Plasmid#26252DepositorInsertmRFPmars
UseTags6xHisExpressionBacterialMutationKpnI restriction site removedPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
p406GALL
Plasmid#17434DepositorInsertGALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALL promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
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