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Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNA13 (GNA13 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(125)DPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα12-133-NLuc
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA12 (GNA12 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position A(133)FPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsS-246-Nluc
Plasmid#207157PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNAS (GNAS Human)
UseTagsExpressionMammalianMutationNLuc inserted at position D(246)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsL-260-NLuc
Plasmid#207158PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNAS (GNAS Human)
UseTagsExpressionMammalianMutationNLuc inserted at position D(260)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ1-LSS-mKATE2(N)
Plasmid#207159PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG1
UseTagsExpressionMammalianMutationN-terminally tagged with LSS-mKATE2PromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus R200W
Plasmid#209179PurposeMammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (R200W) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (R200W)PromoterCMVAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-196)-Venus
Plasmid#209182PurposeMammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.DepositorInsertNKX6-2 (1-196) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus L163V
Plasmid#209178PurposeMammalian expression of SPAX8-related missense mutation (L163V) in human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (L163V) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (L163V)PromoterCMVAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus Y238F
Plasmid#209181PurposeMammalian expression of human NKX6-2 T252D phosphoresistant mutantDepositorInsertNKX6-2 (Y238F) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (Y238F)PromoterCMVAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
S16-ACR1-pcDNA3.1-mScarlet
Plasmid#204963PurposeExpression of S16-ACR1 fused to mScarlet in mammalian cellsDepositorInsertS16-ACR1
UseTagsmScarletExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD
Plasmid#201818PurposeA plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoterDepositorInsertV5-ER-LBD
UseTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-188)-Venus
Plasmid#203714PurposeSPAX8-related truncated mutation (E189*) containing the amino acids 1 to 188 of human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (1-188) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationdeletion of amino acids 189-277PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus S246A
Plasmid#203715Purposehuman NKX6-2 S246A phosphoresistant mutantDepositorInsertNKX6-2 (S246A) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (S246A)PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus S246D
Plasmid#203716Purposehuman NKX6-2 S246D phosphomimic mutantDepositorInsertNKX6-2 (S246D) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (S246D)PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus T252A
Plasmid#203717Purposehuman NKX6-2 T252A phosphoresistant mutantDepositorInsertNKX6-2 (T252A) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (S252A)PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus T252D
Plasmid#203718Purposehuman NKX6-2 T252D phosphomimic mutantDepositorInsertNKX6-2 (T252D) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (S252D)PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin-ILLVtoEEEE_AE
Plasmid#148610PurposeMammalian Expression of DmRoquin-ILLVtoEEEEDepositorInsertDmRoquin-ILLVtoEEEE (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRoquin2_1-500_AE
Plasmid#148615PurposeMammalian Expression of HsRoquin2_1-500DepositorInsertHsRoquin2_1-500 (RC3H2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRoquin2_501-1191_AE
Plasmid#148616PurposeMammalian Expression of HsRoquin2_501-1191DepositorInsertHsRoquin2_501-1191 (RC3H2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only