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Showing: 1561 - 1580 of 3079 results

  1. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  5. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid
    #123362
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    Firefly luciferase
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123362-plasmid-map-sequence-id-240467

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  15. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  16. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

    Plasmid
    #199263
    Purpose
    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199263-plasmid-map-sequence-id-389813

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  18. pSL1777 (pEffector)

    Plasmid
    #160731
    Purpose
    Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins and crRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160731-plasmid-map-sequence-id-318598

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Showing: 1561 - 1580 of 3079 results