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pMK262 (RAD21-mAC Neo)

Plasmid

#140538

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3

Plasmid

#164695

Purpose

Expression of PRMT3 with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3(E338Q)

Plasmid

#164696

Purpose

Expression of PRMT3(E338Q) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E338Q

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pZE-PalkB-GFP

Plasmid

#44381

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Inserts

GFP
PalkB

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PalkB

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pRSF-KpAGO207-1251

Plasmid

#117854

Purpose

Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

Depositor

Kotaro Nakanishi, Dinshaw Patel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Argonaute

Use

Tags

6xHis-SUMO encoded in vector

Expression

Bacterial

Mutation

missing first 206 amino acid residues

Promoter

T7

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSW071

Plasmid

#140037

Purpose

PCC 11901 fadD locus KO with a kanamycin resistance cassette

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Kanamycin resistance gene

Use

Gene knockout in cyanobacteria

Tags

Expression

Mutation

Promoter

KanR

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pG108 - K

Plasmid

#178039

Purpose

Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Insert

Kanamycin resistance

Use

Tags

Expression

Bacterial

Mutation

ErmAM has been replaced with Kanamycin for select…

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDHt-KS

Plasmid

#63616

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pTE1062

Plasmid

#172356

Purpose

Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

Inserts

scbR (SCO6265 )
kasOp

Use

Synthetic Biology; Integrative into streptomyces …

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4R3_T CTP

Plasmid

#171736

Purpose

ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal

Use

Multisite gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1397-DddtoxA-N

Plasmid

#191596

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NI_G1397-DddtoxA-N

Plasmid

#191597

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NN_G1397-DddtoxA-N

Plasmid

#191598

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_HD_G1397-DddtoxA-C

Plasmid

#191583

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NG_G1397-DddtoxA-C

Plasmid

#191584

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_HD_G1397-DddtoxA-N

Plasmid

#191585

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NG_G1397-DddtoxA-N

Plasmid

#191586

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_HD_G1333-DddtoxA-C

Plasmid

#191587

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge