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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Use

Tags

10xHis-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Article

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA7

Plasmid

#26683

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 7 (KPNA7 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Use

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-Merlin

Plasmid

#84293

Purpose

Expression of wt Merlin

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-HaloTag7-CHMP4A

Plasmid

#184895

Purpose

Stable expression of HaloTag7-CHMP4A in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

CHMP4A (CHMP4A Human)

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mNeon

Plasmid

#186598

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-SEC62-FLAG

Plasmid

#128263

Purpose

Expresses SEC62 in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

SEC62 (SEC62 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH60

Plasmid

#139029

Purpose

MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

spe-11 promoter (spe-11 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-citrate

Plasmid

#225251

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-citrate

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Use

Tags

Expression

Mammalian

Mutation

R1608Q

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_1

Plasmid

#78205

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

Depositor

Martin Webb

Article

Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

Insert

matB (His6 C106A R286C Q457C K488A)

Use

Tags

His6

Expression

Bacterial

Mutation

C106A R286C Q457C K488A

Promoter

T7

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Mmp11

Plasmid

#209520

Purpose

Expresses luciferase under control of Mmp11 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Mmp11 promoter (Mmp11 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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CAV1-P132L-mEGFP

Plasmid

#27708

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

P132L

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP1E1D1

Use

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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