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Plasmid#157623PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertKIR2DL5 (KIR2DL5A Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS54 ADAR 3'UTR mut
Plasmid#14488DepositorInsertADAR 3'UTR mutant (ADAR Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3 delta Exon2
Plasmid#100782PurposeMammalian expression of BNIP3 delta Exon2 splice variantDepositorInserthuman-BNIP3 delta Exon2 (BNIP3 Human)
UseTagsHAExpressionMammalianMutationExon2 deletionPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG30 IQGAP1 3'UTR mut
Plasmid#14504DepositorInsertIQGAP1 3'UTR mutant (IQGAP1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGTGCCTT was changed to GTGCGCC and GTGCCT was cha…PromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO NET1A-V5 WT
Plasmid#69828PurposeExpresses NET1A-V5 in mammalian cells in a doxycycline inducible mannerDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseLentiviralTagsv5ExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep70
Plasmid#136870PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep70 (CEP70 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep70
Plasmid#136871PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep70 (CEP70 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1Xpress-BcrGAP R1090A
Plasmid#38176DepositorInsertBCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
UseTagsXpressExpressionMammalianMutationaa 1004-1271 with R1090A point mutationPromoterAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLAP-MIS12-KARD
Plasmid#114057Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertMIS12-KARD (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST NET1A S46A
Plasmid#69818PurposeExpresses NET1A-V5 in mammalian cellsDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseTagsV5ExpressionMammalianMutationS46APromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-301a-3p
Plasmid#103396PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-301a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-301a-3p target (MIR301A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP T271E
Plasmid#73057PurposeC-terminal GFP fusion of Mouse Foxg1 T271EDepositorInsertFoxg1 (Foxg1 Mouse)
UseTagsGFPExpressionMammalianMutationT271EPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP T271A
Plasmid#73047PurposeC-terminal fusion of gfp Mouse Foxg1 T271ADepositorInsertFoxg1 (Foxg1 Mouse)
UseTagsGFPExpressionMammalianMutationT271APromoterCAGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CCR3-DuET
Plasmid#213199PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR3 (CCR3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR(NGS-6)-mCherry
Plasmid#217363PurposeExpresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA mutant, "NGS-6"
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3D-COMP5AP-AviTag-9xHis
Plasmid#157530PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3D (SEMA3D Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ROBO4-COMP5AP-AviTag-9xHis
Plasmid#157428PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertROBO4 (ROBO4 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PSG4-COMP5AP-AviTag-9xHis
Plasmid#157380PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertPSG4 (PSG4 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PSG7-COMP5AP-AviTag-9xHis
Plasmid#157381PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertPSG7 (PSG7 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PSG8-COMP5AP-AviTag-9xHis
Plasmid#157389PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertPSG8 (PSG8 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only