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pCpf1b

Plasmid

#122187

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL3-FLAG

Plasmid

#15820

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin3 (CUL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N418

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ADAR2DD_E488Q

Plasmid

#205598

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

ADAR2 deaminase domain (ADARB1 Human)

Use

Tags

Expression

Mammalian

Mutation

E488Q hyperactive mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-mCherry

Plasmid

#65265

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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mito cpVenus control

Plasmid

#186792

Purpose

Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito cpVenus control

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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alpha Synuclein-VC

Plasmid

#89471

Purpose

expresses human alpha Synuclein fused to the C-terminal part of Venus

Depositor

Tiago Outeiro, Bradley Hyman

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCWX-UBI-hTert-PGK-BSD

Plasmid

#114316

Purpose

Constitutive expression of the hTert protein

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC

Plasmid

#125695

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mScarlet-Gephyrin.FingR-IL2RGTC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

Plasmid

#112022

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha/beta into TRBC1 HDRDT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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