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Plasmid#214139PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2DepositorInsertsUseTags2xSTIIExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pXPR003-sgTP53-4
Plasmid#118022PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-4 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChrimsonR-GFP]
Plasmid#84480PurposeAAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7
Plasmid#214140PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7DepositorInsertsUseTags2xSTIIExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
Plasmid#135814PurposeFluorescent reporter for knock-in of TdTomato into the TH locusDepositorInsertTH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
UseTagsp2a-TdTomatoExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-1
Plasmid#118019PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-1 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.mNeon
Plasmid#69146PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [Chronos-tdTomato]
Plasmid#105834PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-3
Plasmid#118021PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-3 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVZ_PcoaT_SQE1_LUP1
Plasmid#103857PurposeHeterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803DepositorInsertlupeol synthase 1 (LUP1 Mustard Weed)
UseSynthetic BiologyTagsExpressionBacterialMutationinserted genes are codon optimized. 2nd amino aci…PromoterPcoaT (from Synechocystis sp. PCC 6803)Available sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-fd [Chronos-GFP]
Plasmid#84482PurposeAAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPR003-sgTP53-5
Plasmid#118023PurposeConstitutive expression of sgRNA targeting human TP53DepositorInsertsgTP53-5 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]
Plasmid#122103PurposeAAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ENL)
Plasmid#69212PurposeLentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])DepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Chronos-tdTomato]
Plasmid#84481PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-ChrimsonR-GFP
Plasmid#122063PurposeAAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationChrimson K176R mutantPromoterEF1a(1.1kb short version)Available sinceMarch 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Chronos-tdTomato
Plasmid#122099PurposeAAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterEF1α (1.1 kb short version)Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]
Plasmid#108273PurposeAAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationChrimson K176R mutantPromoterEF1α promoter (1.1kb short version)Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-VPR-dSpCas9[StaPL(TI)]
Plasmid#111508PurposeExpresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertVPR-dSpCas9[StaPL(TI)]
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, and S122…PromoterPGKAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]
Plasmid#84485PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α, 1.1 kb long (short version)Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only