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pTRIP-SFFV-mtagBFP-2A-ZebrafishSTING

Plasmid

#196633

Purpose

Express Zebrasfish STING

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

ZebrafishSTING (sting1 Zebrafish)

Use

Lentiviral

Tags

mtagBFP

Expression

Mutation

WT

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HRP-1LC

Plasmid

#139116

Purpose

express the LC of HRP-1

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

181-346

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJ4M_HRP-1_FL

Plasmid

#139117

Purpose

express HRP-1 with a C-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2Bpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM29

Plasmid

#135489

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCitrine

Use

Tags

mCitrine

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM30

Plasmid

#135490

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mClover3

Use

Tags

mClover3

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Use

Tags

mCerulean3

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNB682

Plasmid

#60143

Purpose

Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus-PEST

Use

Tags

PEST degron

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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TagBFP_A1aY1

Plasmid

#158752

Purpose

Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagBFP-T_A1aY1

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mTagBFP2-2A

Plasmid

#102602

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo

Plasmid

#127644

Purpose

Empty pLentiCRISPRv2 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGI3EM20C

Plasmid

#135487

Purpose

Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr H2B-tdTomato

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_anion

Plasmid

#98656

Purpose

Expresses untagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

Plasmid

#184633

Purpose

Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Aldh1a1 (Aldh1a1 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Use

Tags

LifeAct

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2A/Bpr (divergent)

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_K5S

Plasmid

#98679

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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