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Showing: 1541 - 1560 of 1582 results

  8. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

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  9. pcDNA3-CKAR2

    Plasmid
    #123348
    Purpose
    Improved FRET-based biosensor for monitoring Protein Kinase C activity.
    Depositor
    Jin Zhang
    Article
    Ross et al Elife. 2018 Jul 3;7. pii: 35458
    Insert
    CKAR2
    Use
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    123348-plasmid-map-sequence-id-313438

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  11. pRelA*

    Plasmid
    #175590
    Purpose
    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Fused with a flexible GS linker to mVenus
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175590-plasmid-map-sequence-id-348362

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  15. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    FKBP-ICUE3-NLS
    Use
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  16. pMXs-IP- SCAT3.2

    Plasmid
    #172497
    Purpose
    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Amcyan
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Availability
    Academic Institutions and Nonprofits only
    172497-plasmid-map-sequence-id-340999

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  19. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Mutation
    Promoter
    TEF1p
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

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Showing: 1541 - 1560 of 1582 results