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Plasmid#74741PurposepCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpHps2 (extracted from Methylovorus glucosetrophus…Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
NET39 Short pmRFP-N2 (451)
Plasmid#61981Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET39 (PPAPDC3 Human)
UseTagsmRFPExpressionMammalianMutationShort form of NET39PromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP L3-L2
Plasmid#62159PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP R4-R3
Plasmid#62158PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RFP-miR-E2-4int
Plasmid#19824DepositorInsertmiRNA
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP-LC3ΔG
Plasmid#84572PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
Plasmid#113865PurposeTriple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ran-T24N-mRFP1-polyA
Plasmid#104561PurposeExpress Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRan (Ran Mouse)
UseTagsmRFP1ExpressionMammalianMutationT24NPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ran-Q69L-mRFP1-polyA
Plasmid#104560PurposeExpress Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRan (Ran Mouse)
UseTagsmRFP1ExpressionMammalianMutationQ69LPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
Plasmid#190181PurposeFluorescent reporter vector to visualize all of cell cycle phases.DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-miRFP670-pA_U6-BbsI (pE059)
Plasmid#149353PurposeMammalian expression vector for miRFP670DepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LC3-RFP-LC3ΔG
Plasmid#168997PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-ssRFP-GFP-KDEL
Plasmid#128257PurposeFluorescence reporter to monitor ER-phagy activityDepositorInsertssRFPGFPKDEL
UseLentiviralTagsRFPGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAG)20-P2A-RFP
Plasmid#105689PurposeDouble fluorescent stalling reporter K(AAG)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (RFP)
Plasmid#80923PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAA)20-P2A-RFP
Plasmid#105688PurposeDouble fluorescent stalling reporter K(AAA)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only