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Plasmid#77713Purpose3rd generation lentiviral gRNA plasmid targeting human IRAK3DepositorInsertIRAK3 (IRAK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161Q, K163Q
Plasmid#78792PurposeRetroviral expression of p21 K161Q, K163QDepositorInsertP21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161Q, K163Q muationPromoter5'LTRAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS3-MT-BX-6MYC p21 90-164
Plasmid#78786PurposeTo overexpress p21 90-164 in Mammalian CellDepositorInsertP21 (CDKN1A Human)
UseTags6xMYCExpressionMammalianMutationaa90-164PromoterCMV IE94Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS3-MT-BX-6MYC p21 90-139
Plasmid#78785PurposeTo overexpress p21 90-139 in Mammalian CellsDepositorInsertp21 (CDKN1A Human)
UseTags6xMYCExpressionMammalianMutationcontains p21 amino acids 89-139PromoterCMV IE94Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro p21 K161R, K163R
Plasmid#78791PurposeRetroviral expression of p21 K161R, K163RDepositorInsertp21 (CDKN1A Human)
UseRetroviralTagsExpressionMammalianMutationK161R, K163R_muationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCrebbp#2/Cre
Plasmid#193210PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp geneDepositorInsertsgCrebbp#2 (Crebbp Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0881
Plasmid#143445PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXB3 (HOXB3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorUseTags6xHis-TEVExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP core
Plasmid#179543Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertS. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0126
Plasmid#141530PurposeLentiviral vector for overexpressing the CXXC1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCXXC1 (CXXC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-EFa1-HA-Jag1
Plasmid#46051DepositorInsertJag1 (Jag1 Rat)
UseTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_MEGF10_NUP210
Plasmid#205854PurposeExpress mEGFP-tagged fusion protein, MEGF10_NUP210 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-MAFF
Plasmid#192917PurposeBarcoded piggybac transposon vector with Dox-inducible expression of MAFFDepositorInsertMAFF (MAFF Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_GSN_NTRK1
Plasmid#205827PurposeExpress mEGFP-tagged fusion protein, GSN_NTRK1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC17A1
Plasmid#131888PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC17A1 (SLC17A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-GST-FBW7 alpha
Plasmid#197456PurposeThe plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.DepositorInsertFBW7 (FBXW7 Human)
UseIn vitro translationTagsGSTExpressionBacterialMutationSee depositor comments belowPromoterT7Available sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CDKN1A sgRNA
Plasmid#138189Purpose3rd generation lentiviral gRNA plasmid targeting human CDKN1ADepositorInsertCDKN1A (CDKN1A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK6 Q203D
Plasmid#80883Purposemammalian expression of ALK6 Q203DDepositorInsertALK6 (Bmpr1b Mouse)
UseTagsHAExpressionMammalianMutationQ203D (constitutively active)PromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only